| |
τ1 (ns)
|
α1 (%)
|
τ2 (ns)
|
α2 (%)
|
τ3 (ns)
|
α3 (%)
|
τ4 (ns)
|
α4 (%)
|
<τ> (ns)
|
|---|
|
Apo-Tat
|
0.21 ± 0.03
|
25 ± 2
|
1.35 ± 0.01
|
35 ± 3
|
2.60 ± 0.20
|
19 ± 1
|
4.5 ± 0.2
|
21 ± 5
|
1.96 ± 0.08
|
|
Holo-Tat
|
0.22 ± 0.05
|
18 ± 4
|
1.30 ± 0.20
|
37 ± 3
|
2.79 ± 0.09
|
25 ± 3
|
5.1 ± 0.2
|
20 ± 3
|
2.24 ± 0.07
|
- aExperiments were performed with 1.5 μM Tat proteins in 50 mM Hepes buffer, pH7.5, at 20°C. The lifetimes, τ
i
, and relative amplitudes, α
i
, are expressed as means for at least three independent experiments. The mean lifetimes were calculated with: ⟨τ⟩ = ∑α
i
τ
i
. The excitation and emission wavelengths for Trp were set at 295 nm and 350 nm, respectively.
