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Table 1 Fluorescence intensity decay parameters of apo-Tat and holo-Tata

From: Modulation of microtubule assembly by the HIV-1 Tat protein is strongly dependent on zinc binding to Tat

 

τ1 (ns)

α1 (%)

τ2 (ns)

α2 (%)

τ3 (ns)

α3 (%)

τ4 (ns)

α4 (%)

<τ> (ns)

Apo-Tat

0.21 ± 0.03

25 ± 2

1.35 ± 0.01

35 ± 3

2.60 ± 0.20

19 ± 1

4.5 ± 0.2

21 ± 5

1.96 ± 0.08

Holo-Tat

0.22 ± 0.05

18 ± 4

1.30 ± 0.20

37 ± 3

2.79 ± 0.09

25 ± 3

5.1 ± 0.2

20 ± 3

2.24 ± 0.07

  1. aExperiments were performed with 1.5 μM Tat proteins in 50 mM Hepes buffer, pH7.5, at 20°C. The lifetimes, τ i , and relative amplitudes, α i , are expressed as means for at least three independent experiments. The mean lifetimes were calculated with: ⟨τ⟩ = ∑α i τ i . The excitation and emission wavelengths for Trp were set at 295 nm and 350 nm, respectively.
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Retrovirology

ISSN: 1742-4690

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