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Table 1 Fluorescence intensity decay parameters of apo-Tat and holo-Tata

From: Modulation of microtubule assembly by the HIV-1 Tat protein is strongly dependent on zinc binding to Tat

  τ1 (ns) α1 (%) τ2 (ns) α2 (%) τ3 (ns) α3 (%) τ4 (ns) α4 (%) <τ> (ns)
Apo-Tat 0.21 ± 0.03 25 ± 2 1.35 ± 0.01 35 ± 3 2.60 ± 0.20 19 ± 1 4.5 ± 0.2 21 ± 5 1.96 ± 0.08
Holo-Tat 0.22 ± 0.05 18 ± 4 1.30 ± 0.20 37 ± 3 2.79 ± 0.09 25 ± 3 5.1 ± 0.2 20 ± 3 2.24 ± 0.07
  1. aExperiments were performed with 1.5 μM Tat proteins in 50 mM Hepes buffer, pH7.5, at 20°C. The lifetimes, τ i , and relative amplitudes, α i , are expressed as means for at least three independent experiments. The mean lifetimes were calculated with: τ = ∑α i τ i . The excitation and emission wavelengths for Trp were set at 295 nm and 350 nm, respectively.