Figure 1From: Modulation of microtubule assembly by the HIV-1 Tat protein is strongly dependent on zinc binding to TatEffect of zinc binding on Tat oxidation. The number or free -SH groups per Tat molecule was measured according to the Ellman reaction. Tat in NaPi 20 mM buffer, pH6.5 (●), or in Hepes buffer 50 mM, pH7.5, in the absence (△), or in the presence of 2 (■) or 5 (□) zinc equivalents.Back to article page