Skip to main content
Figure 6 | Retrovirology

Figure 6

From: Mouse T-cells restrict replication of human immunodeficiency virus at the level of integration

Figure 6

HIV-1 integration is inefficient in mouse T-cell lines. (A) Human and mouse T-cell lines, infected with VSV-G HIV-CMV-GFP (see also results in Figs. 1–3), were analyzed for levels of HIV-1 integrants using species-specific integration PCRs (Figs. 4, 5 and [6]). Triplicate samples were analyzed. (B) The Relative-Integration-Frequency was calculated as the number of integrants on day 7 p.i. (Fig. 6A) relative to the total amount of HIV-1 cDNA on day 1 p.i. (Fig. 3A) for the identical T-cell infection. -(C) Furthermore, the relative CMV-driven Transgene- Expression-per-Integrant was deduced from the identical experiments and calculated as the ratio of the percentage of GFP-positive cells on day 3 p.i. (Fig. 2B) relative to the number of integrants per ng cellular DNA on day 7 p.i. (Fig. 6A). In (B) and (C) the resulting ratio × 100 is given in arbitrary units, and the arithmetic means ± S.E.M. of 3–5 independent experiments each with duplicates or triplicates are shown.

Back to article page