Figure 4
From: Mouse T-cells restrict replication of human immunodeficiency virus at the level of integration
Establishment of a quantitative PCR for the detection of HIV-1 integrants in the mouse genome. (A) Schematic representation of the strategy and primers for the nested mouse integration PCR using anchor primers specific for highly repetitive mouse B1 elements. A mouse integration standard was generated by infection of mouse NIH3T3 fibroblasts with a low MOI of VSV-G HIV-1NL4-3 GFP, passaging of cells and sorting for GFP-expressing cells 7 weeks p.i. following trichostatin A induction. The resulting cell population was termed NIH3T3int cells. (B) Representative HIV-1 integration standard amplification based on NIH3T3int cells plotted as a function of the natural logarithm of the concentration of HIV-1 (log concentration) versus the values of the PCR cycle threshold. The correlation coefficient R2 and the slope of the correlation are given.