Figure 3
From: Mouse T-cells restrict replication of human immunodeficiency virus at the level of integration
HIV-1 reverse transcription and nuclear import of the pre-integration complex are well supported in T-cell lines and primary T-cells from mice following infection with VSV-G HIV-CMV-GFP. (A, B) The levels of total HIV-1 cDNA and 2-LTR circles were quantified in infected human and mouse T-cell lines, samples being derived from the experiment shown in Figs. 1, 2. One day p.i. cell aliquots were taken and the extracted DNA analyzed for HIV-1 cDNA species and a species-specific gene by real-time PCR as described under Methods. Triplicate samples were analyzed and data are representative for three independent experiments. (C, D) Mitogen/IL-2-activated primary T-cells from two human donors or two pools of splenocytes from 3 BALB/c mice were infected with VSV-G HIV-CMV-GFP BlaM-Vpr and analyzed for (C) virion fusion or (D) 2-LTR circles 6 h and 1d p.i., respectively. Duplicate samples were analyzed.