Figure 5

7SL RNA packaging into HIV-1 virus like particles is dependent on nucleocapsid. A. Schematic representation of HIV-1 VLP-forming GAG construct and its derivative, Zwt-p6. B. VLPs were produced by 293T cells transfection with GAG or Zwt-p6 constructs in the presence of APOBEC3G, APOBEC3F or mock plasmid as indicated. Intracellular and VLP levels of cytidine deaminases and Gag were measured by Western blotting with HA- and capsid-specific antibodies, respectively. C. 7SL RNA in viral like particles was quantified by real time PCR. A representative experiment out of two is shown.