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Figure 1 | Retrovirology

Figure 1

From: Characterization of APOBEC3G binding to 7SL RNA

Figure 1

7SL RNA knockdown does not prevent A3G encapsidation. A. 7SL RNA in cells lines expressing control-shRNA and SRP14-shRNA, was quantified by real time PCR. APOBEC3G or APOBEC3F expressing plasmids were transfected when indicated. Means and standard errors from three independent experiments are shown. B. Production of Vif-defective HIV-1 particles from control-shRNA or SRP14-shRNA cell lines, in the presence of APOBEC3G or APOBEC3F when indicated. Means and standard errors from three independent experiments are shown. C. 7SL RNA, Y3 RNA and 5S RNA measured by real time PCR in Vif defective HIV-1 particles produced from control-shRNA or SRP14-shRNA cell lines, upon transfection of APOBEC3G or APOBEC3F when indicated. Results were normalized to viral genomic RNA. Means and standard errors from three independent experiments are shown. D. Western blot analysis of these viruses and of cytoplasmic extracts of the producer cells, using indicated antibodies, ß-actin and capsid serving as loading controls. E. Infectivity of Vif-defective HIV-1 particles produced from control-shRNA or SRP14-shRNA cell lines, in the presence of APOBEC3G or APOBEC3F when indicated.

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