Effect of blood T. cruzi trypomastigotes and 24 h-supernatant of trypomastigotes on HIV-1 replication in BeWo cells. The human choriocarcinoma BeWo cell line was transducted overnight with VSV-G pseudotyped HIV-1 (V) (100 ng p24/2 × 104 cells per well) alone or in the presence of blood trypomastigotes (BT) (2 × 105 parasites/2 × 104cells per well) or 24 h-supernatant of BT (BTSn). Cells were lysed and luciferase activity as an indicator of viral replication was read from cell lysates at day 4 post-infection or coinfection. Results are expressed as relative light units per second (RLU/sec), presented as a percentage relative to VSV-G. The histogram in red corresponds to the % of infection with VSV-G (= 100%) and the histogram in white corresponds to the % of infection in the presence of BT. Results are representative of 3 independent experiments.