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Figure 1 | Retrovirology

Figure 1

From: Reconstitution of the myeloid and lymphoid compartments after the transplantation of autologous and genetically modified CD34+bone marrow cells, following gamma irradiation in cynomolgus macaques

Figure 1

Schematic representation of SIV-derived SIN vector, helper construct and VSV-g encoding plasmid. An SIVmac251-derived vector was produced by cotransfecting 293T cells with three plasmids: A. a plasmid pGASE containing the eGFP gene under control of the PGK promoter; B. a plasmid pSIV3+ containing viral genes; C. a plasmid pGREV containing the VSV envelope gene. Cis genetic elements are symbolized with white boxes, whereas promoters and genes are depicted by shadowed boxes. pCMV, early cytomegalovirus promoter; pPGK, mouse phosphoglycerate kinase-1 promoter; RRE, REV-responsible element; SA, SIV Rev/Tat splice acceptor; cPPT and PPT, central and 3' polypurine tracks, respectively; GFP, the gene encoding the enhanced green fluorescent protein; LTRsin, partially U3 deleted 3'LTR; LG, leader and a 5' GAG region.

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Retrovirology

ISSN: 1742-4690

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