Variation in HIV-1 R5 macrophage-tropism correlates with sensitivity to reagents that block envelope: CD4 interactions but not with sensitivity to other entry inhibitors

Table 3 R5 envelopes sensitivity to 2G12 neutralization and conservation of critical potential N-linked glycosylation sites.

Envelope		2G12 sensitivity	N295	N332	N339	N386	N392	N448
NA20	B59	+/-	+	+	+	+	+	+
	B76	+	+	+	+	+	+	+
	B501	+/-	+	+	+	+	+	+
	LN3	+	+	+	+	+	+	+
	LN8	+	+	+	+	+	+	+
	LN10	+	+	+	+	+	+	+
	LN14	+	+	+	+	+	+	+
	LN16	+	+	+	+	+	+	+
NA420	B13	-	+	+	-	-	+	+
	B33	-	+	+	-	-	+	+
	B42	+/-	+	+	-	+	+	+
	LN40	+	+	+	-	+	+	+
	LN85	-	+	+	-	+	+	+
NA118	B12	+	+	+	+	+	+	+
	LN27	+	+	+	+	+	+	+
	LN33	+	+	+	+	-	+	+
NA176	B93	+	+	+	+	+	+	+
NA353	B27	-	+	+	+	+	+	+
P-1114	C95-65	+/-	+	+	-	+	+	+
	C96-26	+/-	+	+	+	+	+	+
	C98-15	+	+	+	+	+	+	+
	C98-18	+	+	+	+	+	+	+
	C98-27	+	+	+	+	+	+	+
	C98-28	+	+	+	+	+	+	+
	C98-67	+	+	+	+	+	+	+
P3	Q3 164 1.4	+	+	+	+	+	+	+
	Q3 180 6.4	+/-	+	+	+	+	+	+
	SQ3 196 10.1	+	+	+	+	+	+	+
	SQ3 197 9.3	+	+	+	+	+	+	+
	SQ3 199 8.5	+	+	+	+	+	+	+
P31	Q31 350.1	+/-	+	+	+	+	+	+
	Q31 351.6	+/-	+	+	+	+	+	+
	SQ31 308.2	+/-	+	+	+	+	+	+
P43	Q43 378.2	-	+	+	+	+	+	-
	SQ43 380.1	-	+	+	+	+	+	-
	SQ43 380.4	-	+	+	+	+	+	-
Controls	AD8	+/-	+	+	+	+	+	+
	SF162	+	+	+	+	+	+	+
	YU2	-	+	+	-	+	+	+
	JRFL	+	+	+	+	+	+	+
	JRCSF	+	+	+	+	+	+	+

For 2G12 sensitivity; -, IC50 > 50 μg/ml; +/-, IC50 20–50 μg/ml; +, IC50 < 20 μg/ml.

ISSN: 1742-4690