Variation in HIV-1 R5 macrophage-tropism correlates with sensitivity to reagents that block envelope: CD4 interactions but not with sensitivity to other entry inhibitors

Table 2 Non-parametric two-tailed Spearman analysis for correlations between R5 envelope macrophage-tropism and sensitivity to entry inhibitors.

Inhibitor/Antibody	Target of reagent	Stage of entry blocked³.	Correlation with Macrophage-tropism (p Values)
Q4120	CD4	env: CD4 interactions	<0.0001**
sCD4	gp120, CD4bs	env: CD4 interactions	<0.0001**
PRO 542 (IgG-CD4)	gp120, CD4bs	env: CD4 interactions	<0.0001**
BMS-378806	gp120, CD4bs channel¹.	env: CD4 interactions	0.0002**
b12	gp120, overlapping CD4bs².		0.6843
TAK779	CCR5	env: CCR5 interactions	0.7964
SCH350581	CCR5	env: CCR5 interactions	0.7587
2D7	CCR5	env: CCR5 interactions
2G12	gp120 glycan	env: CCR5 interactions	0.0138*
T20	gp41 conformational changes	gp41 conformational changes	0.7061
2F5	gp41 membrane proximal region	gp41 conformational changes⁴.	0.3741
4E10	gp41 membrane proximal region	gp41 conformational changes⁴.	0.3502

1. BMS-378806 binds to a hydrophobic channel deep in the channel targeted by CD4 [42].
2. Mab b12 binds an epitope that overlaps the CD4bs [57].
3. Mab 2G12 binds to a glycan on gp120. 2G12 blocks env:CCR5 interactions but may also block earlier stages of entry [73].
4. Mabs 2F5 and 4E10 block gp41 conformational changes but may also block earlier stages of entry [73].
* Significant (p ≤ 0.05).
** Highly significant (p ≤ 0.01)

ISSN: 1742-4690