Table 2 MoMLV integrase interacting proteins identified in the yeast two-hybrid screens

Group I, Chromatin binding and transcription factors

Enhancer of zeste homolog 1 (Ezh1/Enx-1/Ezh2)

742/31–292; 31–266; 371–615; 371–641

Polycomb group; chromatin structure maintenance and transcriptional regulation; binds ATRX via SET domain

Transcription factor IIE, beta subunit (TFIIE-β)

292/18–292; 18–228- gap-249–290; 18–233-gap-247–290; 50–292

Subunit of RNA polII holoenzyme; recruits TFIIH to the PolII-TFIIB-TFIID complex

Ku70/XRCC6

608/1–608

NHEJ, chromosome maintenance, 70 kD subunit with Ku80 subunit of DNA-PKcs

Flap endonuclease-1 (Fen1)

381/143–381

Removes 5' initiator tRNA from Okazaki fragments; DNA repair in NHEJ and V(D)J

Tata binding protein ABT1 (ABT1)

269/20–269 (2)

Associates with Tata binding protein and activates basal transcription of class II promoters

B-Activating transcription factor (B-ATF)

120/1–120

AP-1/ATF superfamily; Basic leucine zipper transcription factor; blocks transformation by H-Ras and v-Fos

Bromodomain containing protein 2 (Brd2)/RING3/female sterile homeotic gene-related 1 (fsrg 1)

798/311–543; 357–541; 530–798; 558–798; 560–798; 562–798; 563–798; 594–798; 595–798

Bromodomain-containing protein; interacts with Latency-associated nuclear antigen (LANA-1) of KHSV; mitogen-activated kinase activity; homolog of Drosophila female sterile homeotic gene

All1 fused translocated to Chromosome 9 (AF9)/mixed lineage-leukemia translocated to 3 (Mllt3)

568/238–428, 476–560; 238–428; 182–362

Pc3 interacting protein; Implicated in H3 hypermethylation; YEATS family member (YNL107w/ENL/'AF-9/and TFIIF small subunit)

Bromodomain adjacent to zinc finger domain, 2B (Baz2b)

2123/615–883

Putative member of ISWI containing chromatin remodeling machinery; DDT, PHD-type zinc finger and putative histone acetyltransferase-Methyl-CpG binding domain (HAT-MBD)

Zinc finger p15 (Znfp15)

2192/1526–1808

Binds to Z-box response element between two Pit-1 elements in the growth hormone (GH) promoter; activates GH transcription 100 fold above basal levels

Zinc finger p38 (Znfp38)

555/137–540

Transactivation via SCAN domain; granule cell specification in brain; upregulated in spermatogenesis

Peroxisome proliferative activated receptor, gamma, coactivator-1 related (PRC)

1644/1181–1644; 1321–1644; 1321–1644

Serum-inducible coactivator of nuclear respiratory factor 1- dependent transcription from RNA pol II promoters; stress response protein

Ankyrin rep domain 49 (Ankrd49)

238/6–190

Putative transcription factor; contains acidic activation domain; ankyrin repeat domain is similar to SWI6

Group II, RNA binding proteins

Translation initiation factor 3 (TIF3/eIFs2/TRIP1)

325/128–325 (4)

Translation initiation factor; 5 WD repeats; dissociates ribosomes, promotes initiator Met-tRNA and mRNA binding; yeast homolog TUP12 acts as transcriptional repressor

Splicing factor 3b, subunit 2 (SF3b2)

878/389–844; 385–606; 397–579; 554–781; 397–576

Has putative DNA-binding (bihelical) motif predicted to be involved in chromosomal organization; has SAP domain; proline-rich domain in spliceosome assoc. proteins; basic domain in HLH proteins of MYOD family

Splicing factor 3a, subunit 3 (SF3a3)

501/318–501

Zinc finger, C2H2-type; RNA splicing, mRNA processing

U2 auxiliary factor 26 (U2AF²⁶)

220/53–220

Pre-RNA splicing factor; can replace U2AF³⁵ in vitro

U5 small nuclear ribonucleoprotein (U5 snRNP)

2136/1939–2136

Transcriptional regulation; SNF2 N-terminal domain; conserved C-terminal helicase domain; GTP binding factor; ortholog of S. cerevisiae splicing factor Prp8p; mutations in hPRPC8 are autosomal dominants in retinitis pigmentosum

Step II Splicing factor SLU7

585/27–585

Pre mRNA splicing, required for 3' splice site choice

Survival motor neuron (SMN)

288/12–254

Component of an import snRNP complex containing GEMIN2, 3, 4, 5, 6 and 7; contains one Tudor domain; deficiency leads to apoptosis

Dead box p18 (Ddx18)

660/366–592; 366–610; 366–660; 366–660; 366–590

RNA-dependent helicase; RNA-dependent ATPase activity; stimulated by ss-RNA

Dead box p68 (Ddx68/Ddx5)

615/247–490; 247–510

RNA-dependent helicase and ATPase activity; stimulated by ss-RNA; interacts with HDAC1

Histone stem loop binding protein (HSLbp)

275/1–275; 1–204; 1–248

RNA transcription events, required for histone pre mRNA processing

Group III, Miscellaneous and transport proteins

Ran binding protein 10 (Ranbp10)

503/60–387

Interacts with MET (receptor protein tyrosine kinase) via its SPRY domain; does not interact with SOS, competes with Ranbp9 for MET binding; interacts with Ran in vitro

kinesin super family member 3A (Kif3A)

701/443–701; 443–650

Transport of organelles, protein complexes, and mRNAs in a microtubule- and ATP- dependent manner; chromosomal and spindle movements during meiosis and mitosis

Radixin

389/13–330

Member of ezrin, radixin, moesin family of actin binding proteins. Binds directly to ends of actin filaments at plasma membrane

Transient receptor potential prot.2 (TrpC2)

313/3–313

Calcium ion entry channel; putative involvement in DNA damage response