Host proteins interacting with the Moloney murine leukemia virus integrase: Multiple transcriptional regulators and chromatin binding factors

Table 1 Yeast two-hybrid clone interactions with lexA C-terminal and N-terminal fused MoMLV integrase and with C-terminal fused HIV-1 integrase

Interactions between MoMLV IN, HIV-1 IN, and the clones isolated in the yeast two-hybrid screen. The pACT or pGADNOT plasmids containing the cDNAs isolated from the yeast two-hybrid screens were introduced into strain CTY10-5d bearing either the pSH2-mIN, mIN-pNlexA, or pSH2-hIN plasmids. Qualitative β-galactosidase colony lift assays were performed. No. of isolates in each library: the number of times a clone identified as the indicated insert was retrieved, specific to each library screened. Total number of times an insert corresponding to each protein was retrieved from all screens. Legend: - white; +/- pale blue; + light blue; ++ intermediate blue; +++, ++++ dark blue. Additional controls not shown: pSH2-mLEDGF/pGADNOT-hIN, +++; pSH2-mIN/pGADNOT-mLEDGF, -; pSH2-mLEDGF/pGADNOT-mIN, -.

ISSN: 1742-4690