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Figure 1 | Retrovirology

Figure 1

From: HuR interacts with human immunodeficiency virus type 1 reverse transcriptase, and modulates reverse transcription in infected cells

Figure 1

Identification of HuR as a partner of HIV-1 p66 reverse transcriptase. A. A yeast two-hybrid screen was carried out with HIV-1 RT-p66 as the bait, and a CEMC7 cDNA library as the prey. Amino-acid sequence of HuR and its predicted binding site to HIV-1 p66. RRM: RNA recognition motif. B. Alignment of the different fragments of HIV-1 interacting with HuR in the yeast two-hybrid rebound screen, using HuR as the bait and random fragments of HIV-1 YU-2 isolate as the prey. Numbers in brackets indicate the occurrence of each fragment. C. Mapping of the HuR interaction site on HIV-1 RT bound to a primer-template. Solvent accessible surface (probe radius 1.4 A) of the protein is represented in two different views (PDB 1D 1HMI) [53]. The p51 is shown in blue and p66 in pink. The DNA primer-template is represented in grey. The putative HuR binding site on p66 is represented in red.

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Retrovirology

ISSN: 1742-4690

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