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Table 1 Comparisons of plasma and PBMC genotypes of the PR and RT regions in the HIV-1 infected patient Position in PR or RTa

From: Highly diversified multiply drug-resistant HIV-1 quasispecies in PBMCs: a case report

PR

10

15

16

35

37

39

41

46

48

54

60

62

63

64

68

69

72

77

82

90

     

Plasma

I

I/V

E

D

T

S

 

L

V

V

E

V

P/H

V

 

H/Y

V

I

V/A

      

PBMCs

I

 

E

 

T

S

K/R

I/M

V

I/V

E

V

P

I/V

G/R

H/Y

V

I

V/A

M/I

     

RT

41

43

44

69

98

101

102

108

118

122

162

166

177

181

184

190

203

208

210

211

214

215

219

223

228

Plasma

L

E

A

N

G

K/E

Q

I

I

K

D

R

E

C

V

A

K

Y

W

E

F

Y

R

Q

R

PBMCs

L

E

A

 

G

K/E

Q

 

I

K

H/D

K/R

E

Y/C

V

A/G

K

Y

W

D

F

Y

K/R

Q

R

  1. a: cDNA containing the entire PR and half of RT was synthesized from HIV-1 RNA in plasma by RT-PCR or DNA directly amplified by PCR from proviral DNA of infected PBMCs. DNA was doubly sequenced, visualized and analyzed by the Siemens Automatic System. The sequence of the HIV-1 HxB2 strain was used as the wild type reference strain.