Pvt1-encoded microRNAs in oncogenesis

Retrovirology

Table 3 QPCR measuring mmu-miRNAs encoded by Pvt1.

Tumor #	miR-1204	miR-1205	miR-1206	miR-1207-5p	miR-1207-3p	miR-1208
14	34.54	44.58	BT	29.03	ND	38.96
16	36.35	44.34	36.09	30.39	ND	39.42
20	34.06	42.24	BT	27.92	ND	38.78
21	34.6	39.73	BT	28.39	ND	39.63
22	33.06	41.13	BT	29.6	ND	37.3
23	35.46	42.13	BT	30.07	ND	39.06
31	35.2	39.56	BT	28.43	34.26	38.57
32	35.87	38.65	BT	30.07	34.93	38.9
34	33.3	38.59	BT	28.86	35.3	41.44
35	33.72	42.19	BT	29.91	34.3	ND
36	35.83	40.16	BT	30.85	35.47	39.5
39	33.16	42.44	BT	30.89	35.52	41.3
40	35.05	38.94	BT	31.14	40.16	45.52
41	34.95	43.62	BT	31.15	37.2	40.39
42	34.2	41.75	BT	29.44	35.64	40
43	33.92	40.13	BT	29.45	35.91	40.73
44	36.9	39.98	BT	31.34	36.45	42.1
45	33.36	41.07	BT	27.25	34.72	38.81
46	35.04	42.21	BT	28.93	36.1	41.29
Average ± STD	34.66 ± 1.12	41.23 ± 1.82	ND	29.64 ± 1.17	35.84 ± 1.54	40.09 ± 1.83
C2	38.02	42.23	BT	30.28	36.93	40.53
C3	33.53	41.93	BT	26.98	34.6	39.36
C4	36.19	41.1	BT	29.3	35.45	39.62
C5	36.03	45.22	BT	30.23	ND	39.23
C6.2	ND	37.35	ND	ND	40.31	40.36
C6.3	36.9	42.82	BT	31.77	38.64	41.73
C7	37.08	BT	BT	32.11	ND	41.11
C8	36.51	43.14	BT	30.89	ND	39.55
C9	37.15	44.73	BT	30.86	ND	40.32
C 10	36.8	45.2	BT	30.4	ND	39.26
Average ± STD	36.47 ± 1.25	42.64 ± 2.47	BT	30.31 ± 1.51	37.19 ± 2.33	40.11 ± 0.85
thymus	35.01	34.41	BT	33.06	38.17	43.4
spleen	34.05	40.3	BT	31.03	36.4	41.97
Reject H0	yes	no		no	no	no

Numbers in the first column correspond to the tumour numbers in the Table 1; other columns represent the cycle numbers needed to reach a predetermined threshold. BT, below threshold; ND, not done; STD, standard deviation. A cycle number of 30 corresponds to approximately 3000 molecules per cell.

ISSN: 1742-4690