HIV-1 latency in actively dividing human T cell lines

Retrovirology

Table 1 Overview of SupT1 clones with integrated HIV-rtTA provirus

Clone	HIV-rtTA gDNA PCR	HIV-1 resistant¹	Dox-induced				Number detected proviruses
			HIV-rtTA replication²	Intracellular CA-p24³
				(24 h)	(48 h)	M.F.I.⁵
B5	+	-	-	0.01	0.04	-	-⁴
B8	+	-	-	0.38	0.34	62	-
C2	+	+	-	0.04	0.00	-	1
C4	+	+	-	0.01	0.00	-	-
C7	+	+	-	0.00	0.00	-	2
D2	+	+	-	0.01	0.06	-	1
D10	+	-	-	0.01	0.02	-	-
D11	+	+	-	0.02	0.01	-	1
E4	+	+	-	0.00	0.01	-	2
F3	+	-	-	0.00	0.01	-	-
H7	+	+	-	0.00	0.00	-	3
B4	+	+	+	0.38	0.50	70	1
B6	+	+	+	0.22	0.19	78	5
B9	+	+	+	1.34	1.66	69	3
C5	+	+	+	0.72	0.82	106	3
C11	+	+	+	0.01	0.04	-	3
D4	+	+	+	0.07	0.02	-	4
D6	+	+	+	0.07	0.02	-	1
E7	+	+	+	0.43	0.59	100	-
E11	+	+	+	0.05	0.02	-	-
F7	+	+	+	10.52	11.54	49	2
F8	+	+	+	1.31	2.16	82	1
F9	+	-	+	0.07	0.02	-	1
G2	+	+	+	0.02	0.01	-	1

¹ Wild-type HIV-1 LAI superinfection
² HIV-rtTA replication scored by syncytia formation and extracellular CA-p24 production
³ Percentage CA-p24 positive cells
⁴ -; not detected
⁵ M.F.I. is the mean fluorescence intensity of the CA-p24 positive cells (48 h dox)

ISSN: 1742-4690