Figure 5

Latent HIV-1 and HIV-rtTA infection in SupT1 T cells. TNFα-induced reactivation of silent HIV-1 proviruses in SupT1 cells was analyzed by determining the percentage of CA-p24 producing cells by intracellular FACS analysis. After a single round infection with HIV-1 (LAI isolate) or the HIV-rtTA-Tat^wt, each culture was split and either activated for 24 h with TNFα or not. The fold TNFα activation is the percentage CA-p24 positive cells in the culture with TNFα divided by the percentage of CA-p24 positive cells in the control culture.