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Figure 2 | Retrovirology

Figure 2

From: HIV-1 latency in actively dividing human T cell lines

Figure 2

Northern blot analysis of shRNA production in rtTA-shNef SupT1 cell lines. RNAs from the different HIV-rtTA-shNef SupT1 clones were isolated and separated on an agarose gel. After blotting the membrane was probed with a LNA probe against the shNef. Lane M, marker; lane 1, shNef clone B9; lane 2, clone C10 (a negative control cell line without a provirus); lane 3, shNef clone D2; lane 4, shNef clone D11; lane 5, shNef clone F7; lane 6, shNef clone F8; lane 7 and 8 contain positive controls from a transfection with the F-shNef- and F-shNef+ plasmid [31]; lane 9 contains a negative empty-vector control; lane 10 in vitro produced shNef RNA.

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