Figure 6

Selective reduction of HIV-1-infected T cells by the lentiviral vector carrying anlO. One million CEM-SS cells were first infected with the replication-competent virus NL4-3.HSA.R+ (250 ng p24 per million). Aliquots of the infected cells were then superinfected 24 hours later with two different doses of vNL-AnlO-RRE-SA (for the 1 × dosage, m.o.i. 0.5 – 1). The extent of spreading HIV infection was measured by mouse CD24 (HSA) staining using a PE-labeled rat monoclonal antibody against HSA. Flow cytometry analyses were performed at day 5, 7, and 9 post HIV infection. Shown are the mouse CD24 staining (HSA) (y-axis) and the Forward Scatter (FSC) (x-axis) of the cells. The second dose of vNL-AnlO-RRE-SA was 100 fold (100 ×) higher than the first one (1 ×). Uninfected cells (Cell) were similarly stained and used as a control.