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Figure 6 | Retrovirology

Figure 6

From: HIV transfer between CD4 T cells does not require LFA-1 binding to ICAM-1 and is governed by the interaction of HIV envelope glycoprotein with CD4

Figure 6

The role of adhesion molecules in HIV transmission between primary CD4 T cells. In panel A the expression of the indicated adhesion molecules was analyzed in the surface of T cells from a representative NL4-3 infected PBMC culture. Cells were gated as CD3+/CD8-/CD4+ PBMCs (non-productively infected cells, upper histograms) or CD3+/CD8-/CD4- PBMCs (productively infected cells, lower histograms). Histograms show a representative experiment displaying the expression of each individual antigen (solid peaks) with the negative control of staining (empty peaks). In panel B, purified productively HIV infected CD3+/CD8-/CD4- PBMCs were cocultured with CMFDA-labeled primary unstimulated CD4 T cells. After 24 hours of coculture cells were stained with anti-CD45RO and anti-HIV CA p24 antibodies. HIV transmission was measured in both memory CD45RO+ (RO+ solid bars) and naive (RO-, empty bars) subsets in the presence of Leu3a, C34 and a panel of blocking agents against adhesion molecules used at the same concentrations as in Figure 3 (whole IgGs against ICAM-1, LFA-1 and ICAM-3, soluble ICAM-1 or Fab fragments of the anti-ICAM-3 mAb 140.11). Values are Mean ± SD of data corresponding to up to 6 different donors. Asterisks indicate significant differences (p < 0.05) from control or between divalent and monomeric blocking agents.

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