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Figure 9 | Retrovirology

Figure 9

From: Distinct efficacy of HIV-1 entry inhibitors to prevent cell-to-cell transfer of R5 and X4 viruses across a human placental trophoblast barrier in a reconstitution model in vitro

Figure 9

A soluble factor secreted by CD4 negative cells from PBMCs contributes to the increase of viral passage through the BeWo cell monolayer by T20. Purified CD4+ T-cells were infected with HIV-1/LAI and used as effector cells, as in the experiment from figure 8. Different media were used as diluent for cells and T20 during the contact with CD4 negative BeWo cells. The media used were either normal culture medium (M) (RPMI 1640 medium), or in 1) 24 h culture supernatant of HIV-1 LAI infected PBMCs, 2) 24 h culture supernatant of uninfected PBMC or 3) 24 h culture supernatant of CD4 negative cells from PBMCs. The figure shows p24 Ag produced in basolateral chamber at day 8 post contact in these different conditions. The histograms are means (with standard deviations to the means) of values from three different wells of one experiment out of two.

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