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Figure 3 | Retrovirology

Figure 3

From: Distinct efficacy of HIV-1 entry inhibitors to prevent cell-to-cell transfer of R5 and X4 viruses across a human placental trophoblast barrier in a reconstitution model in vitro

Figure 3

The fusion inhibitors T20 and C34 enhance viral passage across the BeWo cell monolayer in contact with PBMCs infected with X4 HIV-1. PBMCs infected with HIV-1 LAI or HIV-1 KH025 isolates were pre-incubated with varying doses of T20 or C34 for 30 minutes. Then, HIV-1 LAI or HIV-1 KH025 infected PBMCs were added to the BeWo cell monolayer and were left in contact for 3 h at 37°C. Extensive washes were then performed and the Transwell® inserts transferred on new plates containing activated HIV negative indicator PBMCs, without drugs. The inserts were removed 5 days later and the culture continued 3 more days, when the HIV-1 p24 core antigen was quantified in the supernatants. The figures show HIV-1 passage across the model of the trophoblast barrier normalized to the control (without drugs) as a function of T20 (or C34) concentration. Continuous line, CD4 positive BeWo cells as target cells; dashed line: CD4 negative BeWo cells as target cells. 3A: PBMCs infected with HIV-1 LAI as effector cells in the presence of T20; 3B: PBMCs infected with HIV-1 KH025 as effector cells in the presence of T20 and 3C: PBMCs infected with HIV-1 LAI as effector cells in the presence of C34.

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