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Figure 1 | Retrovirology

Figure 1

From: Distinct efficacy of HIV-1 entry inhibitors to prevent cell-to-cell transfer of R5 and X4 viruses across a human placental trophoblast barrier in a reconstitution model in vitro

Figure 1

Basal HIV-1 p24 antigen production by indicator PBMCs in the basolateral chamber of the in vitro trophoblast barrier model. After 3 h contact between PBMCs infected with the indicated viral isolates (effector cells) and BeWo cells (targets), the Transwell® inserts were extensively washed and left in culture in plates containing activated, HIV-1 negative, indicator PBMCs for 5 days. Inserts were then removed, and cultures of indicator PBMCs were continued for 3 more days and HIV-1 p24 core antigen was assayed in the supernatants. The figure shows the average (mean ± SD) of at least 2 independent experiments performed in triplicates. The grey bars represent target CD4 negative BeWo cells and the black bars represent target CD4 positive BeWo cells. The asterix on the top of the bars denotes a statistically significant difference (p = 0.02) by Mann-Whitney U test.

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