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Figure 6 | Retrovirology

Figure 6

From: Extracellular ATP reduces HIV-1 transfer from immature dendritic cells to CD4+T lymphocytes

Figure 6

ATP affects virus survival within iDC. iDCs were initially pulsed for 60 min at 37°C with NL4-3Balenv in absence or presence of ATP (400 μM). Next, cells were washed extensively, treated with trypsin to remove uninternalized viruses and then either lysed immediately (A) or incubated at 37°C for the indicated time periods (B). The viral contents found either intracellularly and in cell-free supernatant were assessed using a p24 test. The data shown represent the means ± standard deviations of triplicate samples and are representative of three independent experiments. In panel B, the upper part of each bar represents p24 recovered in the supernatant, whereas the intracellular p24 content is shown in the lower part of each bar. (C) iDCs were either left untreated or pretreated with NH4Cl (20 mM) for 30 min. Next, iDCs were pulsed for 60 min at 37°C with NL4-3Balenv in absence or presence of the indicated concentrations of ATP. Cells were washed extensively to remove input virus and excess ATP and co-cultured with autologous CD4+ T cells at a 1:3 ratio. Viral production was assessed two days following initiation of the co-culture. The data shown represent the means ± standard deviations calculated from four independent experiments with triplicate samples and are presented as relative viral production of ATP-treated cells compared to control cells for each condition. The asterisk denotes statistically significant data (*, P < 0.05).

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