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Figure 4 | Retrovirology

Figure 4

From: Extracellular ATP reduces HIV-1 transfer from immature dendritic cells to CD4+T lymphocytes

Figure 4

Extracellular ATP affects the early transfer phase. (A) iDCs were initially either left untreated or treated with 50 nM efavirenz. Next, cells were either left untreated or treated with ATP before addition of NL4-3Balenv. Finally, cells were washed and co-cultured with autologous CD4+ T cells for 2 days before assessing viral production. The percentage of inhibition is shown above the appropriate bars. (B) Transfer studies were carried out using recombinant luciferase-encoding viruses pseudotyped with the JR-FL envelope. Luciferase activity was assessed at 3 days following initiation of the co-culture. (C) iDCs were pulsed for 60 min with NL4-3Balenv in absence or presence of ATP (400 μM). Next, iDCs were extensively washed to remove input virus and cultured in complete culture RPMI medium supplemented with GM-CSF and IL-4. Cell-free supernatants were collected and assayed for the p24 content at the indicated days post-infection. Data shown represent the means ± standard deviations of triplicate samples and are representative of three (panels A and C) or two (panel B) independent experiments.

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