Figure 4

Toxicity of lime on cervical epithelial cells. Cervical epithelial cells (ME180) were exposed to LiJ (diluted using normal saline) for 5 (red circles), 30 (blue circles) or 60 (green circles) minutes. Juice was then removed by washing and tissue viability assessed by the method of MTT dye reduction. Data shown are expressed as percentage viability (when compared to an untreated control) andrepresent the mean ± SEM of n = 4 independent experiments where each condition was tested in quadruplicate. The 50% toxic dose (TD₅₀) of LiJ was determined using non-linear regression analysis.