Figure 4

P. gingivalis increases infectious HIV-1 associated with oral keratinocyte plasma membrane and intracellular fractions. TERT-2 cells with or without pre-incubation with P. gingivalis were inoculated with R5- (Ba-L) (A and C) or X4-tropic (IIIb) (B and D) HIV-1. The protocol is as described in the Materials and Methods and summarized in the legend of Fig. 1. After washing, cells were trypsinized to recover membrane-associated (A and B) and cell-associated, trypsin-resistant infectious HIV-1 (C and D). To assay for infectious HIV-1 virions, virus-containing fractions were incubated with TZM-bl cells, stained with X-Gal and positive blue cells counted as described in the Materials and Methods. Data represent the mean ± SEM of TZM-bl positive cells from two independent experiments, each in triplicate.