Amplification | Primers | HXB2 Coordinate | Sequence (5'-3') |
---|---|---|---|
N-term 3.0 kb fragment | |||
First round | N558F | 0001 | TGGAAGGGTTAATTTACTCTAAGGAAAGGCAAGAGATCCTTG |
 | N453R | 4820 | CTATTATGTCTACTATTCTTTCCCCTGCAC |
Second round | N533F | 0001 | GGGGGGCAGTGCGGCCGCTGGAAGGGTTAATTTACTCYMAGAAAAGRCAAG |
 | N502R | 2878 | TGAAAAATACGCGTCMCCCACATCCAGTACTGT |
C-term 6.5 kb fragment | |||
First round | N501F | 1851 | CCAYAAAGCACGCGTKTTGGCTGARGCAATGAG |
 | N476R | 9719 | TGCTAGAGATTTTCCACACTGACTAAAAGGGTCTGAGGTCTCTAGTT |
Second round | N503F | 2841 | ACTGGATGTGGGKGACGCGTATTTTTCAGTTCC |
 | N600R | 9719 | GCTTCGCATCCTGCAGGTGCTAGAGATTTTCCACACTGACTAAAAGGGTC |