Longitudinal microarray analysis of cell surface antigens on peripheral blood mononuclear cells from HIV+ individuals on highly active antiretroviral therapy

Table 2 Changes over time in the expression of cell surface antigens (p < 0.05) on PBMC from HIV+ individuals treated with highly active antiretroviral therapy

Antigen	Baseline	TimePoint2	TimePoint3	TimePoint4	P value^a
CD2	124 (103–147)	126 (107–156)	130 (84–136)	144 (129–163)	0.047
CD3	70 (59–86)	70 (52–90)	69 (43–85)	94 (85–117)	0.007
CD5	90 (76–120)	89 (76–129)	88 (75–106)	121 (98–137)	0.040
CD36	44 (29–63)	43 (34–56)	61 (40–77)	62 (45–75)	0.017
CD95	101 (89–115)	100 (95–121)	99 (95–121)	121 (97–131)	0.032
CD27	30 (19–40)	37 (18–60)	20 (11–57)	47 (35–72)	0.015
CD28	61 (33–81)	81 (55–92)	65 (47–80)	81 (71–95)	0.007

Data are presented as median dot intensities (i.e., cell binding densities) quantified using DotScan data analysis software on an 8-bit pixel grey scale from 0–255. The 25^th–75^th quartile values are shown in brackets. The cut off value of 10 was used for the detectable expression levels.
a Level of significance of the time parameter in a repeated measure mixed model analysis of variance was used to assess the changes in cell binding density values over time.

ISSN: 1742-4690