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Table 1 Effects of Rtm on the fusion activity of Env protein.

From: Characterization of a new 5' splice site within the caprine arthritis encephalitis virus genome: evidence for a novel auxiliary protein

Plasmida

No. of syncytia/well (293T/GSM cocultivation)b

% of syncytiac

pKCR3

0

0

pKEnv

954 ± 105

100

pKEnv + pKRtm

200 ± 46

21

  1. aThe plasmid amounts were normalized by addition of empty plasmid pKCR3 in order to have the same copy number of SV40 promotor in all transfections.
  2. bNumbers of syncytia in 30 randomly selected fields per well. Averages of three wells are shown.
  3. cNumber of syncytia divided by the number obtained with the positive control (pKEnv + pKCR3 plasmids) × 100.