Figure 7
Rtm binds to the cytoplasmic domain of Env protein. Purified recombinant GST-CD™ or GST alone were incubated with in vitro-translated Rtm protein labeled with [35S]methionine. The beads were washed six times in the presence of different ionic strengths of KCl, as indicated, and the bound proteins were subjected to 15% SDS-PAGE analysis and autoradiography.