Figure 5

Identification of a novel CAEV ORF. The 617-bp cDNA amplified by nested-PCR from CAEV-infected GSM cells (see Fig. 4B) was cloned and sequenced. The region sequenced (uppercase letters) is bound by primers MarN and M3b (overlined) used in the second-round PCR. The region in lowercase letters is from the previously published CAEV nucleotide sequence. Numbers in brackets indicate the nucleotide positions of the CAEV genomic sequence (22). The predicted translation product (named Rtm) is shown below the sequence. The amino acids shared by the Rev and Rtm proteins are boxed. The nucleotide and amino acid substitutions of the cDNA compared to the previously published CAEV-Cork sequence are underlined. Deletion is represented by an open triangle. Stop codon is designated as asterisk.