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Table 4 Effect of various inhibitors of glycosylation on virus infectivity in M. dunni and NIH 3T3 cells

From: Role of receptor polymorphism and glycosylation in syncytium induction and host range variation of ecotropic mouse gammaretroviruses

  

Log10 Virus Titer/Inhibitora

Virus

Cells

None

DMM

Sw

2DG

CST

Tu

Spl574

NIH3T3

3.1

4.2

4.4

4.6

4.2

4.1

 

M. dunni

5.7

5.8

NT

5.8

NT

5.8

MoMLV

NIH 3T3

5.7

NT

NT

5.9

NT

5.5

 

M. dunni

2.7

4.4

4.7

4.9

4.1

4.5

  1. aVirus was quantitated using the XC overlay assay [9] and numbers represent XC PFU in 0.2 ml. Glycosylation inhibitors were added the day before virus infection as follows: deoxymannojirimycin (DMM, 100 ug/ml); castanospermine (CST, 100 ug/ml), swainsonine (Sw, 200 ug/ml), 2-deoxy-D-glucose (2DG, 25 mM), tunicamycin (Tu, 0.2 ug/ml). NT, not tested.