Role of receptor polymorphism and glycosylation in syncytium induction and host range variation of ecotropic mouse gammaretroviruses

Table 2 Titers of ecotropic LacZ pseudotypes in mouse cells and MA139 cells transfected with mCAT-1 (FerrM) or dCAT-1 (FerrD2)

	Log₁₀ Titer of LacZ Pseudotypes^a
Cells^b	FrMLV	Spl574	MoMLV
NIH 3T3	3.8	2.7	4.0
M. dunni	2.7	4.5	---
FerrM (mCAT-1)	3.8	4.4	4.1
FerrD2 (dCAT-1)	4.2	4.9	4.2

^aMeasured as the number of cells positive for β-galactosidase activity in 100 ul. ---, no positive cells in cultures infected with 0.1 ml of undiluted pseudotype stock. Bold figures represent titer reduced by more than 95% (1.3 log₁₀) relative to susceptible NIH 3T3 or M. dunni cells.
^bCAT-1 gene expressed in transfected cells is given in ().

ISSN: 1742-4690