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Figure 4 | Retrovirology

Figure 4

From: Apoptosis resistance in HIV-1 persistently-infected cells is independent of active viral replication and involves modulation of the apoptotic mitochondrial pathway

Figure 4

Caspase-3 activation by H 2 O 2 and STS treatment in uninfected and HIV persistently-infected lymphoid cell lines. A) H9, H9/HTLVIIIB, Jurkat and J1.1 cells were exposed to H2O2 and STS. After 24 h, cells were washed and lysed with RIPA buffer. Equal amounts of protein (30 μg/sample) were separated on a 10% SDS-PAGE and blotted onto nitrocellulose membranes. Blots were probed with anti-procaspase-3 for 1 h and revealed with a peroxidase-conjugated anti-IgG antibody and ECL (enhanced chemoluminiscence) Equal loading was checked by analyzing β-actin expression (data not shown). B) H9 and H9/HTLVIIIB cells were exposed to 10μM H2O2, 0.1 μM STS or complete medium for 24 h and collected to evaluate active caspase-3 by PE-conjugated monoclonal anti-active caspase-3 antibody by flow cytometry. C) Jurkat and J1.1 cells were exposed to 10 μM H2O2, 0.1 μM STS, 40 ng/ml CH11 or complete medium for 24 h and collected to evaluate active caspase-3 by PE-conjugated monoclonal anti-active caspase-3 antibody by flow cytometry as described in Materials and Methods

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