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Table 4 ABCB4 co expression increases the PC/SM ratio of lentiviral vector particles

From: Alteration of viral lipid composition by expression of the phospholipid floppase ABCB4 reduces HIV vector infectivity

 

ABCB4 mutant, virus (n = 3)

ABCB4 wild type, virus (n = 3)

ABCB4 mutant, 293T (n = 2)

ABCB4 wild type, 293T (n = 2)

PC/PE

1.3 ± 0.3

1.8 ± 0.4

1.8 ± 0.1

1.6 ± 0.1

PC/SM

2.1 ± 0.6

4.1 ± 0.7*

6.5 ± 4

7.8 ± 5

  1. Lipids were extracted from virus and from membranes of virus producing cells. The ratio's of phosphatidylcholine (PC) phosphatidyl ethanolamine (PE) en sphingomyelin (SM) were determined by TLC as described. * p < 0.05 vs ABCB4 mutant virus.
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Retrovirology

ISSN: 1742-4690

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