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Table 4 ABCB4 co expression increases the PC/SM ratio of lentiviral vector particles

From: Alteration of viral lipid composition by expression of the phospholipid floppase ABCB4 reduces HIV vector infectivity

  ABCB4 mutant, virus (n = 3) ABCB4 wild type, virus (n = 3) ABCB4 mutant, 293T (n = 2) ABCB4 wild type, 293T (n = 2)
PC/PE 1.3 ± 0.3 1.8 ± 0.4 1.8 ± 0.1 1.6 ± 0.1
PC/SM 2.1 ± 0.6 4.1 ± 0.7* 6.5 ± 4 7.8 ± 5
  1. Lipids were extracted from virus and from membranes of virus producing cells. The ratio's of phosphatidylcholine (PC) phosphatidyl ethanolamine (PE) en sphingomyelin (SM) were determined by TLC as described. * p < 0.05 vs ABCB4 mutant virus.