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Table 1 Co-expression of wild type ABCB4 reduces viral infectivity.

From: Alteration of viral lipid composition by expression of the phospholipid floppase ABCB4 reduces HIV vector infectivity

  TU/mla p24/mla TU/pg p24
Empty vector (n = 6) 100 % 100 % 9.9 ± 2.8**
ABCB4 wild type (n = 8) 17.0 ± 9.0 % 90.5 ± 55.3 % 2.3 ± 0.7
ABCB4 mutant (n = 8) 88.9 ± 39.8 %* 104.6 ± 30.3 % 8.9 ± 2.8**
ABCC1 (n = 7) 106.1 ± 48.4 %** 119.3 ± 66.9 % 7.8 ± 1.1**
  1. PGKGFP virus was produced with wild type ABCB4, mutant ABCB4 or with ABCC1. The amount of p24 per ml and the viral titers were determined. Co expression of ABCB4 during viral production significantly reduces viral vector infectivity.
  2. aThe data for TU/ml and p24/ml are presented as a percentage, with the co-transfection of empty vector during the production of lentiviral vectors given an arbitrary value of 100%. Significant difference p < 0.01* or p < 0.001** compared to ABCB4 co-expression. Mean values are presented ± SD.