Figure 3From: Alteration of viral lipid composition by expression of the phospholipid floppase ABCB4 reduces HIV vector infectivityVSVg incorporation in viral particles. Virus produced with wild type and mutant ABCB4 was concentrated and VSVg protein was detected by Western blotting. When equal amounts of HIV p24 were loaded on the gel, the intensity of VSVg staining is also identical. With loading of equal amounts of transducing units (TU) the intensity of VSVg staining is much stronger in the virus produced with ABCB4. This indicates that ABCB4 expression during production of viral vectors does not compromise VSVg incorporation.Back to article page