Figure 3
From: Human prostate supports more efficient replication of HIV-1 R5 than X4 strains ex vivo
HIV-1 R5, R5X4 and X4 infection of human prostate in organotypic culture. RT activity was measured in supernatants of human prostate explants exposed to HIV-1 R5SF162 (n = 3 donors) (A), R5X492US723 (n = 3 donors) (C), or X4IIIB (n = 5 donors) (E); and in supernatants of activated PBMCs exposed to day 17 supernatants of prostate explant cultures exposed to R5SF162 (n = 3) (B), R5X492US723(n = 3) (D) or X4IIIB (n = 5) (F). RT activity was never detected in the supernatants of PBMCs infected with an inoculum of the respective viral stocks maintained at 37°C in medium for 17 days. Each dot represents the mean ± SEM of three to five independent cultures (Dunnett test; *, P < 0.05; control: day 7).