Figure 3

pEGFP-miRNA construct expressing hsa-mir-29a and 29b. (A) Diagrammatic representation of pEGFP-N3 vector containing pre-miR29a and 29b. (B) Expression of hsa-mir-29a and 29b confirmed by quantitative real-time PCR. Data show the expression levels of mir-29a and 29b from pEGFP-miR29a and 29b constructs compared to pEGFP-N3 vector. The data represent mean ± S.D. from two independent experiments in triplicates and have been normalized to mir-92 levels. (C) GFP expression from pEGFP-miRNA transfected HEK293T cells. Upper panel represents GFP (+)ve cells, while the lower panel shows total number of cells transfected with pEGFP-N3 (left), pEGFP-miR29a (middle) and pEGFP-miR29b (right), under the 10× objective of a NIKON florescent microscope.