Table 1 Theoretical pIs of unmodified and modified RT containing phosphorylation or deamidations of 6His-tagged recombinant RTLAI (rRT) [37], RTHXB2 (Swiss-Prot: P04585), and GAPDH [42].
Theoretical isoelectric point (pI) | ||||||
|---|---|---|---|---|---|---|
Protein | Unmodified | No. of Phosphorylation groups | Deamidations | |||
| Â | Â | 1 | 2 | 3 | 1 | 2 |
rRT LAI p66 | 8.53 | 8.16 | 7.60 | 7.19 | 8.36 | 8.13 |
rRT LAI p51 | 8.60 | 8.17 | 7.44 | 7.02 | 8.41 | 8.13 |
RT HXB2 p66 | 8.53 | 8.19 | 7.55 | 7.09 | 8.36 | 8.12 |
RT HXB2 p51 | 8.60 | 8.21 | 7.56 | 7.07 | 8.43 | 8.18 |
GAPDH | 8.52 | 7.54 | 7.0 | 6.71 | 8.25 | 7.82 |