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Figure 1 | Retrovirology

Figure 1

From: Human Immunodeficiency Virus type-1 reverse transcriptase exists as post-translationally modified forms in virions and cells

Figure 1

2D gel electrophoresis analysis of recombinant RT identifies protein isoforms. Recombinant RTLAI + GAPDH protein (3 μg each) was solubilised in 2D gel electrophoresis buffer, focussed on a pH 7–11 non-linear, 11 cm Immobiline DryStrip gel then resolved on a 10% acrylamide SDS-PAGE gel followed by transfer to PVDF membranes. RT was detected by Western blot using an anti-RT antibody (upper panel) and GAPDH detected by Coomassie stain (lower panel). RT isoforms are designated by black arrows and calculated pI indicated. Position of triangles (Δ) denote the reference marks used for calculation of pI.

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Retrovirology

ISSN: 1742-4690

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