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Figure 8 | Retrovirology

Figure 8

From: Caspase-3-mediated cleavage of p65/RelA results in a carboxy-terminal fragment that inhibits IκBα and enhances HIV-1 replication in human T lymphocytes

Figure 8

Binding affinity assay of p65wt-tag and ΔNH 2 p65-tag to IκBα by using in vitro translated proteins. (a) One microgram of in vitro translated p65wt-tag, ΔNH2p65-tag and IκBα were analyzed by immunoblotting using the anti-FLAG tag M2 mAb and an antibody against IκBα. (b) The immunoprecipitation assays of p65wt-tag and ΔNH2p65-tag proteins – alone or combined at different rates – were carried out using a polyclonal antibody against IκBα. Immunoblotting was performed with monoclonal antibodies anti-FLAG and anti-IκBα (10B). Gel bands were quantified by densitometry and background noise was subtracted from the images. Relative ratio of optical density units was calculated regarding to the gel band with less optical density for each condition.

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