Skip to main content
Figure 1 | Retrovirology

Figure 1

From: Caspase-3-mediated cleavage of p65/RelA results in a carboxy-terminal fragment that inhibits IκBα and enhances HIV-1 replication in human T lymphocytes

Figure 1

Subcellular localization of a p65/RelA amino-truncated form in activated human T cells. (a) Human PHA-treated PBLs were incubated in presence of LMB or PMA for 4 and 2 hours respectively. Ten micrograms of cytosolic and nuclear protein extracts were analyzed by immunoblotting (IB) using specific antibodies against IκBα and the carboxy-terminus of p65/RelA. Major cleaved form of p65/RelA is indicated with a black arrow, whereas a minor truncated form is indicated by an arrow with discontinuous line. (b) A hundred micrograms of cytosolic and nuclear protein extracts from Figure 1a (input) were subjected to immunoprecipitation (IP) with an antibody against IκBα and then analyzed by immunoblotting using specific antibodies against IκBα and either carboxy- or amino-terminus of p65/RelA. (c) Human PHA-treated PBLs were incubated in the presence of PMA or TNFα for 2 hours. A hundred micrograms of nuclear protein extracts were subjected to immunoprecipitation with an antibody against IκBα and then analyzed by immunoblotting using specific antibodies against the carboxy-terminus of p65/RelA.

Back to article page

Retrovirology

ISSN: 1742-4690

Contact us