Figure 3

Activation of the galectin-1 promoter by Tax expression in transfected T cell lines. A. Jurkat cells were transfected with either pNF-κB-Luc, pHTLV-Luc or pSRE-Luc (7.5 μg) along with pHβPr.1neo (control vector) or expression vectors for Tax WT, Tax M22 or Tax 703 (7.5 μg) and pActin-LacZ (5 μg). B,C. Jurkat, CEM-T4 and Sup T1 T cell lines were co-transfected with pHβPr.1neo (control vector) or expression vectors for Tax WT or Tax M22 (7.5 μg), the galectin-1 promoter reporter constructs pGL3-gal-1 0.5 kb (B) or pGL3-gal-1 1.2 kb (C) (7.5 μg) and pActin-LacZ (5 μg). D. Jurkat cells were transfected with pNF-κB-Luc or pGL3-gal-1 1.2 kb (15 μg). After transfection (24 hours), cells were either left untreated or stimulated with PMA or TNF-α for 8 hours. Luciferase and β-galactosidase activities were determined 48 hours after transfection as described in Materials and Methods. In panels A, B and C, luciferase activity was normalized on the basis of the β-galactosidase activity. The results represent the mean of three independent transfections +/- standard deviations (*p < 0.05; **p < 0.01).