Figure 7
Electron microscopy of viral-like particles generated by the R1A and P8A cell lines. (A) Images of virus particles from R1A cell lines. Single virus-like particles were present at the cell membrane (left panel) and at an intracellular vacuole (middle panel). Immuno-gold image was obtained after labeling with an antibody specific for the virus CAgag protein (right panel) (Scale bar = 400 nm). (B) Images of the virus particles from P8A cell lines. Multiple virus-like particles were present at the cell membrane (left panel) and in intracellular vacuoles (middle panel). Immuno-gold image was obtained after labeling with anti-CAgag antibody (right panel) (Scale bar = 400 nm). (C) Quantification of virus particles and of labeling of viral particles with gold beads. The average number of viral particles was 1.85/μm2 and 8.85/μm2 in the R1A and P8A cell lines, respectively. The average number of gold particles was 9.98/μm2 and 24.28/μm2 in the R1A and P8A cell lines, respectively. *statistically significant difference (p < 0.01). (D) Negative stain images of single viral particle from R1A and P8A cell lines. R1A-viral particle size averaged 70 nm and P8A-viral particle averaged 80 nm large.