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Figure 2 | Retrovirology

Figure 2

From: Physiological properties of astroglial cell lines derived from mice with high (SAMP8) and low (SAMR1, ICR) levels of endogenous retrovirus

Figure 2

Establishment of astroglial cell lines immortalized by SV40 T antigen. (A) Confirmation of SV40 large T antigen (105 bp) in R1A, P8A, and ICR-A cell lines by PCR analysis. 100 bp M: 100 bp DNA ladder marker; Zpl 2-1: a positive control; SAMR1 and SAMP8 brain: negative controls. (B) Characterization of cell types by Western blot analysis. SAMP8 brain: a positive control for GFAP (50.0 kDa) and NeuN (66.0 kDa); Zpl 2-1: a positive control for NeuN and a negative control for GFAP.

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