Figure 7
The N386 carbohydrate is not essential for DC-mediated HIV-1 transmission. A. LuSIV cells were incubated with the two viruses and luciferase was measured after 24 hours. RLU, relative light units. Error bars represent standard deviations. B. DCs were incubated for 2 hours with wt or N386D virus, followed by extensive washing to remove unbound virus. Viral capture was subsequently determined by lysis of the cells and CA-p24 ELISA. C. DCs were incubated for 2 hours with wt or N386D virus, followed by extensive washing to remove unbound virus. DCs were subsequently cocultured with LuSIV cells to allow HIV-1 transmission. Transmission efficiency was determined by measuring luciferase activity after 24 hours.