Figure 6From: The carbohydrate at asparagine 386 on HIV-1 gp120 is not essential for protein folding and function but is involved in immune evasionNeutralization in single cycle infection assays. Wt an d N386D HIV-1LAI virus and wt and N386D pseudovirus derived from HIV-1JR-FL were preincubated with antibody and subsequently incubated with TZM-bl cells containing a luciferase reporter construct under control of the HIV-1 LTR as described in the materials and methods section. The luciferase activity in the absence of inhibiting reagents was set at 100%.Back to article page